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. Author manuscript; available in PMC: 2021 Jul 24.
Published in final edited form as: J Cell Sci. 2017 Jan 3;130(4):791–804. doi: 10.1242/jcs.187914

Fig. 1. A high-content screen uncovers regulators of peroxisome size and number.

Fig. 1

(A) Schematic representation of the high-content screen workflow. A query strain containing the peroxisomal marker Pex3-mCherry and the ER marker Spf1-GFP was crossed with strain collections of gene deletions and hypomorphic alleles. Following selection, the resulting haploid yeast each contained both a single mutation and the markers. The libraries were imaged using a high-throughput automated microscope. Images were analyzed by software-assisted single-cell and object (peroxisome) recognition, allowing the identification of mutants that show aberrant peroxisome size or number. (B) Distribution of the Z score of the mean peroxisome size for successfully analyzed mutant strains. Mutants of peroxisomal membrane proteins are marked in red, and other peroxisomal proteins marked in black. (C) Distribution of the Z score of the mean peroxisome number per cell for successfully analyzed mutant strains. A high enrichment of peroxisomal membrane protein mutants and Δpex mutants is found in the group of decreased peroxisome number, as illustrated by the inset pie chart, presenting functional classes of the 30 mutants in this group. YGR168C is a previously uncharacterized gene found within this group. Mutants of peroxisomal membrane proteins are marked in red, and other peroxisomal proteins are marked in black.