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. Author manuscript; available in PMC: 2021 Jul 24.
Published in final edited form as: J Cell Sci. 2017 Jan 3;130(4):791–804. doi: 10.1242/jcs.187914

Fig. 2. Deletion or overexpression of YGR168C affects peroxisome number and function.

Fig. 2

(A) Examples of mutants from the high-content screen showing aberrant peroxisome content. Images of strains from the high-content screen containing Pex3-RFP and Spf1-GFP. Peroxisome size is increased in Δvps1, and peroxisome number is decreased in Δvps1, Δpex12 and Δygr168c, compared to a wild-type (WT) strain. Scale bar: 5 μm. Graph shows quantification of these parameters (see also Fig. S1). n>72, ***P<0.01 compared with WT (t-test). (B) Reduction in peroxisome number in Δygr168c is independent of the peroxisomal marker used. The number of peroxisomes per cell was analyzed by using several peroxisomal membrane or matrix proteins tagged with GFP, either in a WT orΔygr168c background. The decrease in peroxisome number in Δygr168c is seen with all peroxisome markers used, n=6. ***P<0.01 (Δygr168c versus WT; t-testforeach marker). (C) Overexpression of YGR168C reduces thenumberofperoxisome puncta. Images of strains with Pex3-GFP and that are WT, Δygr168c or overexpress YGR168C (OE-YGR168C). Scale bar: 5 μm. (D) Peroxisome number is affected in ygr168c mutants grown in glucose and oleate. Quantification of mean cell peroxisome number per cell in WT, Δygr168c, or OE-YGR168C cells, visualized aftergrowth in either glucose (gray) or oleate (red) medium. While an increase in peroxisome number is observed in oleate compared to glucose foreach strain, peroxisome number is decreased in the mutants compared to WT in glucose medium, and in OE-YGR168C in oleate. n>73l. ***P<0.01 compared with the same medium for WT (t-test). All quantitative data are mean±s.e.m.