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. Author manuscript; available in PMC: 2021 Jul 24.
Published in final edited form as: J Cell Sci. 2017 Jan 3;130(4):791–804. doi: 10.1242/jcs.187914

Fig. 3. STED microscopy reveals characteristics of wild-type and ygr168c peroxisomes.

Fig. 3

(A-C) Examples of STED super-resolution microscopy of wildtype (WT; A), Δygr168c (B) and strains overexpressing YGR168C (OE-YGR168C; C) expressing EYFP-SKL as a peroxisomal marker and labeled with anti-GFP nanobodies. Scale bar: 200 nm. The size of peroxisomes was determined as the FWHM of a Gaussian fit. Dashed lines, positions of size and cluster measurements. Peroxisome diameter in Δygr168c is not altered. Overexpression of YGR168C causes an increase in the apparent size of peroxisomes. In conventional light microscopy, these aggregates appear as enlarged single peroxisomes. Sub-diffraction imaging reveals that enlarged peroxisomes consist of multi-lobular structures, whereby the individual substructures appear as peroxisomes of wild-type-like morphology. The distances between the maxima varies between 137 and 174 nm. (D) The mean diameter of WT peroxisomes is 174±8 nm (s.e.m.). The size difference between wild-type and knockout peroxisomes 96±7 nm (s.e.m.) was not significant (P=0.1). The mean diameter of OE-YGR168C peroxisome clusters is 285 nm (±16 nm s.e.m.) (***P=6×10-8 compared with WT). n>50 in all cases.