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. Author manuscript; available in PMC: 2021 Jul 24.
Published in final edited form as: J Cell Sci. 2017 Jan 3;130(4):791–804. doi: 10.1242/jcs.187914

Fig. 5. Pex35 is found in proximity to peroxisomal membrane proteins as well as to Arf proteins.

Fig. 5

(A) A protein complementation assay for Pex35 interactions reveals a physical proximity to the Pex11, Pex25 and Arf1. Query strains with Pex35 tagged with either the C- or N-terminal fragment of the DHFR enzyme were mated with collections of strains expressing the other half of DHFR in the opposing mating type. Interaction between the Pex35 (bait) and a screened protein (prey) results in complementation of the DHFR enzyme, which in turn enables growth on the methotrexate medium, as shown for Ant1 and Pex19. (B) Pex35 is in proximity to Pex11, Pex25 and Arf1. Each of the three indicated Pex35-prey combinations, shown in the center of each image, display large colonies, in contrast to the neighboring colonies with other Pex35-prey combinations. (C) Summary of proteins in proximity to Pex35. Fold increase colony size: the maximum colony size of four combinations of the DHFR screen (overexpression or native promoter for PEX35 bait, and two DHFR fragment collections).