Table 2. Top hits of the PEX35 synthetic genetic interactions screen.

Rank	Gene	Protein	SM	DM	DM/SM
1	YOR130C	Ort1	0.86	0.24	0.27
2	YKL184W	Spe1	0.67	0.19	0.29
3	YNL268W	Lyp1	0.13	0.04	0.34
4	YCR028C-A	Rim1	0.12	0.05	0.39
5	YDR234W	Lys4	0.81	0.32	0.40
6	YJL101C	Gsh1	0.22	0.11	0.47
7	YBR081C	Spt7	0.20	0.10	0.48
8	YJL071W	Arg2	0.21	0.11	0.51
9	YNL270C	Alp1	1.30	0.69	0.53
10	YER116C	Slx8	0.25	0.14	0.54
11	YGL143C	Mrf1	0.49	0.28	0.57
12	YBR251W	Mrps5	0.13	0.07	0.57
13	YNL005C	Mrp7	0.30	0.17	0.58
14	YHR147C	Mrpl6	0.19	0.11	0.61
15	YER185W	Pug1	1.51	0.94	0.62
16	YGL076C	Rpl7a	0.49	0.31	0.63
17	YMR142C	Rpl13b	0.49	0.32	0.65
18	YPR099C	Ypr099c	0.30	0.20	0.66
19	YDR028C	Reg1	0.37	0.24	0.66
20	YCL058C	Fyv5	0.67	0.44	0.66
21	YJL088W	Arg3	0.37	0.25	0.68
22	YDR448W	Ada2	0.36	0.24	0.68
23	YOL108C	Ino4	0.47	0.32	0.69
24	YOL095C	Hmi1	1.22	0.84	0.69
25	YDL077C	Vam6	1.06	0.73	0.69
26	YBR196C-A	Ybr196c-a	0.40	0.28	0.69
27	YDR017C	Kcs1	0.82	0.57	0.69
28	YBR282W	Mrpl27	0.45	0.31	0.69
29	YJR118C	Ilm1	0.74	0.51	0.69
30	YNL252C	Mrpl17	0.28	0.20	0.69

SM, normalized colony size of single mutant; DM, normalized colony size of double mutant; DM/SM: colony size ratio of the double to single mutant.