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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nat Struct Mol Biol. 2021 Jun 10;28(6):512–520. doi: 10.1038/s41594-021-00605-6

Extended Data Fig. 7. Proposed ping-pong reaction mechanism for ELOVL7.

Extended Data Fig. 7

a, Transacylation step with acyl chain of first substrate being transferred to H150. In the second step, malonyl-CoA binds and undergoes decarboxylation and a condensation reaction to form the elongated 3-keto product. b, Proposed reaction steps leading to C-N covalent adduct with H150 seen in crystal structure. In this scenario a 2,3-trans-enoyl-CoA serves as the first substrate (left) leading to the 3-keto,4,5-trans-enoyl-CoA ‘product’ (middle) which subsequently crosslinks to H150 via a conjugate addition reaction of H150 (right). The nature of the reaction that leads to H181 crosslinking to the C2 atom of the 3-keto-acyl-CoA is not clear.