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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: J Hepatol. 2018 Aug 22;69(6):1231–1241. doi: 10.1016/j.jhep.2018.08.012

Fig. 2. Kinetics of the HBV-DNA forms, viral RNA, and protein expression in HepG2-NTCP-K7 cells.

Fig. 2

(A-F) HepG2-NTCP-K7 cells were infected with HBV at an moi of 1,000 vp/cell. At indicated time points, (A) DNA extracted after Hirt lysis and (B) intracellular capsid-associated DNA, and (C) total RNA were detected by Southern or northern blot analysis, respectively, using an HBV-DNA probe. (D) Extracellular HBV-DNA was quantified by qPCR. (E) Intracellular HBx, core, and surface proteins were detected by western blot analysis. (F) Secreted HBsAg and HBeAg were quantified by immunoassay. (G) Each band or value determined at different time points in panel A to F was quantified and plotted. Values from 15 dpi were set to 100%. dpi, day post-infection; HBeAg, HBV e antigen; HBsAg, HBV surface antigen; HBV, hepatitis B virus; moi, multiplicity of infection; qPCR, real-time PCR; SM, size marker; vp, virus particle.