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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: J Hepatol. 2018 Aug 22;69(6):1231–1241. doi: 10.1016/j.jhep.2018.08.012

Fig. 5. Evaluation of cell-to-cell transmission of HBV.

Fig. 5

(A) HepG2-GFP-Rab7 cells and HBV-infected HepG2-NTCP-K7 cells were co-seeded into a 24-well plate at three different ratios and co-cultured. (B) HepG2-NTCP-K7-GFP-Rab7 cells and HBV-infected HepG2-NTCP-K7 cells were co-cultured at three different ratios in the continuous presence of neutralizing antibodies (NAb; 0.5 IU/ml Hepatect CP). After 21 days, cells were fixed, stained with an anti-core antibody, and analyzed by immunofluorescence staining. GFP signal was visualized in parallel. HBV, hepatitis B virus.