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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Protoc. 2021 Mar 26;16(4):2213–2256. doi: 10.1038/s41596-021-00497-2

Fig. 2. Outline of in vitro 3D cardiac microtissues (MTs) formation and analysis.

Fig. 2

Graphical illustration describing MT generation from frozen stocks of ECs, CFs and CMs and characterization of structural and functional properties using: immunofluorescence, contraction and calcium transient analysis, Seahorse analysis, sharp electrode electrophysiology and single-cell dissociation.