Table 3. Variability table of morphological, contraction and single-cell electrophysiological parameters.
The table reports quantitative parameters showing the expected sample-sample, batchbatch and line-line reproducibility. Size: diameter of MTs mounted on coverslips; IBI: inter-beat interval; RMP: resting membrane potential; APA: action potential amplitude; Vmax: maximal upstroke velocity; APD90: action potential duration at 90% repolarization; SD: standard deviation; CV: coefficient of variation (calculated as SD/mean*100). These data have been previously shown in 11. Raw data are reported in Supplementary Table 1.
| Morphology | Contraction | Single-cell electrophysiology | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Size (μm) | Sarcomere length (μm) | Sarcomere alignment index | IBI (ms) | Contraction duration (normalized) | RMP (mV) | APA (mV) | Vmax (dV/dT) | APD90 (ms) | ||
|
Sample to
sample variability |
mean | 482.4 | 1.78 | 0.027 | 3631.4 | 393.9 | -71.0 | 102.4 | 52.8 | 254.9 |
| SD | 38.3 | 0.06 | 0.004 | 819.3 | 118.1 | 6.7 | 10.7 | 32.3 | 89.2 | |
| CV | 7.8 | 3.1 | 13.4 | 22.1 | 31.8 | 9.4 | 10.5 | 61.5 | 33.8 | |
| Batch to batch variability | mean | 471.4 | 1.78 | 0.027 | 3334.2 | 386.6 | -71.2 | 102.9 | 49.4 | 277.1 |
| SD | 31.0 | 0.02 | 0.002 | 1347.9 | 140.7 | 2.3 | 4.2 | 9.3 | 31.8 | |
| CV | 6.8 | 1.3 | 6.6 | 35.9 | 35.4 | 3.2 | 41 | 16.3 | 10.6 | |
| Line to line variability | mean | 471.4 | 1.78 | 0.026 | 3331.2 | 393.5 | -71.5 | 102.9 | 49.9 | 272.0 |
| SD | 46.7 | 0.004 | 0.000 | 584.2 | 24.4 | 1.1 | 0.8 | 11.8 | 68.6 | |
| CV | 9.9 | 0.2 | 0.02 | 17.5 | 6.2 | 1.5 | 0.8 | 23.7 | 25.2 | |