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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Chem. 2021 Jun 10;13(8):805–810. doi: 10.1038/s41557-021-00711-4

Figure 3. X-ray irradiation mediated activation of azido coumarin.

Figure 3

(a) Irradiation of 7-azido-4-methylcoumarin 8 resulting in the generation of 7-amino-4-methylcoumarin 9 with fluorescence turn-on. (b) HPLC traces of the reaction mixture of 8 (RT = 4.57 min) after irradiation with 0 Gy to 60 Gy to afforded product 9 (RT = 3.21 min). (c) Graph showing the yield of 9 generated with different irradiation doses. The data are presented as mean ± SD (n = 3). (d) Flow cytometry analysis (λex/em = 360/450 nm) of cells incubated with 8 (100 μM) with 9 generated in cells following 0 Gy, 6 Gy, 36 Gy and 60 Gy irradiation. Forward versus side scatter profiles were used to gate intact cellular materials. Flow cytometry analyses are based on a population of 10,000 cells.