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. Author manuscript; available in PMC: 2022 Jan 23.
Published in final edited form as: Sci Immunol. 2021 Jul 23;6(61):eabg5003. doi: 10.1126/sciimmunol.abg5003

Figure 5. Activation of immediate-early genes in response to BCR signaling.

Figure 5

(A,B) Pax5-independent (A) and Pax5-dependent (B) immediate-early genes that were induced > 9-fold upon anti-IgM stimulation in control FO B cells and were further defined, as described in Fig. S5D-F and the Materials and Methods. The expression of activated genes in Cd23-Cre Pax5 fl/+ (fl/+; blue) and Cd23-Cre Pax5 fl/− (fl/–; red) FO B cells before (0 h) and after (1 h) of anti-IgM stimulation is shown as mean expression value (TPM) with SEM based on two independent RNA-seq experiments for each genotype and treatment condition. (C) mRNA expression of Pax5-dependent immediate-early genes, coding for known transcription factors, is shown for FO B cells of the indicated genotypes after 1 h of anti-IgM stimulation. Genes bound by Pax5 (12) are underlined. (D) Intracellular Myc staining of Cd23-Cre Pax5 fl/+ (Pax5 Δ/+) and Cd23-Cre Pax5 fl/− (Pax5 Δ/−) FO B cells before (gray) and after (black) stimulation for 1 h with anti-IgM (left). Myc expression (right) is shown as mean MFI value with SEM and was analyzed by twoway ANOVA with Tukey’s multiple comparison test; ****P < 0.0001. Each dot represents one mouse.