Fig. 5. VM-IAP-associated gene expression is altered in Mtrr^gt/gt mice.

Left-hand graphs assess the correlation between VM-IAP methylation and adjacent Slc15a2 (a), Eps8l1 (b), and Gm13710 (c) gene expression in C57BL/6J livers (n = 8, r: Pearson’s correlation coefficient; p: two-tailed P value associated with r). Centre graphs show qRT-PCR expression data of VM-IAP-neighboring genes Slc15a2 (a), Eps8l1 (b), and Gm13710 (c) in C57BL/6J (n = 8, grey circles) and Mtrr^gt/gt (n = 8, red circles) liver. P values were calculated by two-tailed Welch’s t-tests (means shown as black lines). Right-hand graphs incorporate both control and Mtrr^gt/gt data and assess the correlation between gene expression and VM-IAP methylation (n = 16, r: Pearson’s correlation coefficient; p: two-tailed P value associated with r). Relative expression was normalized to Hprt1 expression and calculated using the ΔCt method. Gene Diagrams of VM-IAPs in relation to their neighboring gene are depicted on the far left. Gene transcripts, extracted from the University of California, Santa Cruz (UCSC) Genome Browser⁷², are shown in black and VM-IAPs in purple. Green arrows represent the location of qRT-PCR primers. Diagrams are drawn to scale. See also Extended Data Figure 3.