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. Author manuscript; available in PMC: 2022 Feb 19.
Published in final edited form as: Cell Chem Biol. 2021 Feb 10;28(8):1169–1179.e6. doi: 10.1016/j.chembiol.2021.01.008

Figure 5. LC lyso-PSs robustly cause histamine release from primary mast cells.

Figure 5

(A) Representative microscopy image showing degranulation of PCMCs after 1a (C16:0 lyso-PS) or (R)-2d (C16:0) treatment (both 1 㭜M, 30 mins, 37 °C) in the presence of concanavalin A (Con-A) (black bar = 100 㭜m). (B) Histamine release profile from PCMCs treated with 1a (C16:0 lyso-PS) or (R)-2d (C16:0) or (S)-2d (C16:0) (1 nM to 10 mM, 30 mins, 37 °C, n = 3/data point). (C) Dose response and EC50 values for histamine release from PCMCs following treatment with the different (R)-Me-lyso-PSs (1 nM to 10 mM, 30 mins, 37 °C, n = 3/data point).