Table 1. Kinetic constants for lyso-PS and Me-lyso-PS substrates tested against hABHD12 (HEK293T membrane lysates transiently transfected with hABHD12).

Lyso-PS species	Fatty acid chain length: unsaturation	Vmax (nmol/mg protein/min)	KM (μM)	Vmax/K M (nmol/mg protein/min/M) (x 105)
1a	16:0	15.6 ± 0.6	72 ± 8	2.2 ± 0.3
1b	18:0	18.3 ± 0.5	48 ± 4	3.8 ± 0.4
1c	18:1	17.8 ± 0.6	46 ± 6	3.8 ± 0.5
(R)-2a	10:0	2.9 ± 0.2	126 ± 18	0.2 ± 0.04
(R)-2b	12:0	7.6 ± 0.3	131 ± 14	0.6 ± 0.06
(R)-2c	14:0	12.2 ± 0.6	106 ± 14	1.1 ± 0.1
(R)-2d	16:0	15.2 ± 0.6	67 ± 7	2.3 ± 0.3
(R)-2e	18:0	17.2 ± 0.6	47 ± 5	3.7 ± 0.5
(R)-2f	20:0	19.0 ± 0.6	43 ± 4	4.4 ± 0.5
(R)-2g	22:0	22.0 ± 0.6	40 ± 4	5.5 ± 0.6
(R)-2h	24:0	22.9 ± 0.6	40 ± 4	5.7 ± 0.6
(S)-2a	10:0	0.2 ± 0.02	275 ± 32	0.007 ± 0.001
(S)-2b	12:0	0.4 ± 0.05	255 ± 35	0.016 ± 0.002
(S)-2c	14:0	0.6 ± 0.1	215 ± 28	0.028 ± 0.003
(S)-2d	16:0	0.8 ± 0.1	187 ± 24	0.043 ± 0.006
(S)-2e	18:0	1.2 ± 0.1	178 ± 25	0.067 ± 0.008
(S)-2f	20:0	1.3 ± 0.2	165 ± 23	0.078 ± 0.009
(S)-2g	22:0	1.2 ± 0.2	167 ± 26	0.072 ± 0.009
(S)-2h	24:0	1.2 ± 0.3	176 ± 31	0.068 ± 0.008