Extended Data Figure 2. Senseless expression and growth in wingless null patches surrounded by wild type or NRT-Wingless-expressing cells.
a, b, Expression of Senseless (red) is lost in patches of wingless mutant cells (GFP-negative; wgCX4 homozygotes) except in the cells located within one cell diameter of surrounding GFP-positive cells, which are wild type cells in (a) or homozygous wg[KO; NRT-Wg] in (b) (white arrows). Mosaics were created by mitotic recombination in a way that generates approximately the same number progenitors for the two genotypes, as described in Methods. c, Example of mosaic imaginal discs generated as above to measure the growth of wingless mutant territory (wgCX4 homozygous; GFP-negative) relative to that of wild type (c) or wg[KO; NRT-Wg] homozygous (d) tissue. Wingless and NRT-Wingless, detected with anti-Wingless, are shown in red. e, Outline of the territory where the surface areas were assessed. f, Quantification of the areas colonised by wingless mutant cells (GFP-negative) in the two genetic backgrounds. On average, the wingless null territory was smaller in the wg[KO; NRT-Wg] homozygous background (n=24) than in the wild type (n=20, *** = p <0.001). Error bars represent standard deviation. Statistical significance was assessed by Student’s t-test.