| Primer | Sequence, 5’ > 3’ |
|---|---|
| Forward, 5’ arm | GATCAGTGCGGCCGCGCCGAGAAGAGATCGCCACCACCACTCTACTCTTTTGCACATGCC |
| Reverse, 5’ arm | GATCGCTAGCCGGCACACACACTCTCACACTGACACACGGGGTATGATAGATACTTCC |
| Forward, 3’ arm | GATCATGCATGGACACTGCCCGCCTCCAGCCCAGTCCCGTCCTCTGAAGCCGCCC |
| Reverse, 3’ arm | GATCCCTAGGGCCGATCTGTTGCAATTTCCAAATCAAACAGCGCGCGAAACGTGTGGC |
| For confirmation of 5’ recombination (genomic, forward) | CAGCACTAAAATGGCTTCCTCCGC |
| For confirmation of 5’ recombination (vector, reverse) | CAACTGAGAGAACTCAAAGG (within attP site) |
| For confirmation of 3’ recombination (vector, forward) | TCGTATAATGTATGCTATACG (within Gal4 Poly A) |
| For confirmation of 3’ recombination (genomic, reverse) | GTTCCCGGAATAGTTTAGACCTC |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.