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. Author manuscript; available in PMC: 2021 Aug 25.
Published in final edited form as: Nat Biomed Eng. 2021 Jul 8;5(8):897–913. doi: 10.1038/s41551-021-00757-2

Fig. 5. Ductal subcluster-specific genes located within PLDOs and primary pancreas tissue on the protein level.

Fig. 5

a, PDLOs after 23, 27, and 31 DOD were stained for duct-like cluster 1 marker CFTR, and duct-like cluster 2 marker MUC13, respectively. b, PDLOs differentiated until day 28 stained for cell subtype markers identified by the scRNA- seq analysis. c,d, The same markers are used to locate the in-vitro-generated duct-like cell subtypes in primary human pancreas tissue, either (c) healthy or (d) pancreatitis tissue. Images are complemented by Supplementary Fig. 7. Scale bar denotes 50 μm.