REAGENT or RESOURCE Antibodies	SOURCE	IDENTIFIER
Antibodies
InVivoMAb anti-mouse CD16/CD32 (Clone 2.4G2)	Bioxcell	Cat # BE0307
Fixable Viability Dye eFluor® 780	eBioscience	Cat # 65-0865-18
Anti-mouse CD3e Biotin (Clone 145-2C11)	BioLegend	Cat# 100304
Anti-mouse CD5 Biotin (Clone 53-7.3)	BioLegend	Cat# 100604
Anti-mouse CD19 Biotin (Clone 6D5)	BioLegend	Cat# 115504
Anti-mouse Gr1 Biotin (Clone RB6-8C5)	BioLegend	Cat# 108404
Anti-mouse F4/80 Biotin (Clone BM8)	BioLegend	Cat# 123106
Anti-mouse CD11b Biotin (Clone M1/70)	BioLegend	Cat# 101204
Anti-mouse CD11c Biotin (Clone N418)	BioLegend	Cat# 117304
Anti-mouse NK1.1 Biotin (Clone PK136)	BioLegend	Cat# 108704
Anti-mouse TCRβ Biotin (Clone H57-597)	BioLegend	Cat# 109204
Anti-mouse TCRγδ Biotin (Clone eBioGL3)	eBioscience	Cat # 13-5711-85
Anti-mouse FceR1 Biotin (Clone MAR-1)	BioLegend	Cat# 134304
Anti-mouse TER-119 Biotin (Clone TER-119)	BioLegend	Cat# 116204
Anti-mouse CD45R/B220 Biotin (Clone RA3-6B2)	BioLegend	Cat# 103204
Anti-mouse Ly6G Biotin (Clone 1A8)	BioLegend	Cat# 127604
Streptavidin V500	BD Biosciences	Cat# 561419
Anti-mouse Thy1.2 FITC (Clone 30-H12)	BioLegend	Cat# 105306
Anti-mouse Thy1.2 AF786 (Clone 30-H12)	BioLegend	Cat# 105331
Anti-mouse CD127/IL7Rα APC (Clone A7R34)	BioLegend	Cat# 135012
Anti-mouse CD127/IL7Rα BV605 (Clone A7R34)	BioLegend	Cat# 135041
Anti-mouse CD25 PE-Cy7 (Clone PC61)	BioLegend	Cat# 102016
Anti-mouse ICOS PE-Cy7 (Clone C398.4A)	BioLegend	Cat# 313520
Anti-mouse/human KLRG-1 FITC (Clone 2F1/KLRG1)	BioLegend	Cat# 138410
Anti-mouse/human KLRG-1 Percp-Cy5.5 (Clone 2F1/KLRG1)	BioLegend	Cat# 138417
Anti-mouse PD1/CD279 PE-Cy7 (Clone 29F.1A12)	BioLegend	Cat# 135216
Anti-mouse PD1/CD279 FITC (Clone 29F.1A12)	BioLegend	Cat# 135213
Anti-mouse CCR6 PE (29-2L17)	BioLegend	Cat# 129804
Anti-mouse CD103 FITC (2E7)	BioLegend	Cat# 121420
Anti-mouse CD45.1 FITC (Clone A20)	BioLegend	Cat# 110706
Anti-mouse CD45.2 BV711 (Clone 104)	BioLegend	Cat# 109847
Anti-mouse CD45.2 Pacific Blue (Clone 104)	BioLegend	Cat# 109820
Anti-mouse CD45 AF-700 (Clone 30-F11)	BioLegend	Cat# 110724
Anti-mouse CD3 AF-700 (Clone 17A2)	BioLegend	Cat# 100215
Anti-mouse CD3 BV711 (Clone 17A2)	BioLegend	Cat# 100241
Anti-mouse IL-5 PE (Clone TRFK5)	BioLegend	Cat# 504304
Anti-mouse IL-17A AF700 (Clone TC11-18H10.1)	BioLegend	Cat# 506914
Anti-mouse IL13 AF488 (Clone TWAJ)	eBioscience	Cat # 53-7133-82
Anti-mouse GATA-3 eF660 (Clone TWAJ)	eBioscience	Cat # 50-9966-42
Anti-mouse GATA-3 PerCP-eF710 (Clone TWAJ)	eBioscience	Cat # 46-9966-42
Anti-mouse Eomes AF488 (Clone Dan11mag)	eBioscience	Cat # 53-4875-82
Anti-mouse IL25R/IL17RB eF660 (Clone MUNC33)	eBioscience	Cat # 50-7361-82
Anti-mouse IL18Rα PE-Cy7 (Clone P3TUNYA)	eBioscience	Cat # 25-5183-82
Anti-mouse NKp46 / CD335 PercP-eF710 (29A1.4)	eBioscience	Cat # 46-3351-82
Anti-mouse/human TCF1/7 AF488 (Clone C63D9)	Cell Signalling	Cat # 6444S
Anti-mouse IL33R/ST2 BV421 (Clone U29-93)	BD Biosciences	Cat# 566309
Anti-mouse RORγt PE (Clone Q31-378)	BD Biosciences	Cat# 562607
Anti-mouse RORγt AF647 (Clone Q31-378)	BD Biosciences	Cat# 562682
Anti-mouse RORγt BV650 (Clone Q31-378)	BD Biosciences	Cat# 564722
Anti-mouse PLZF PE (Clone Q31-378)	BD Biosciences	Cat# 564850
Anti-mouse Ki67 PerCP-Cy5.5 (Clone B56)	BD Biosciences	Cat# 561284
Anti-mouse CTLA-4 PE (Clone UC10-4B9)	BD Biosciences	Cat# 553720
Anti-mouse CD121b PE (Clone 4E2)	BD Biosciences	Cat# 554450
Anti- mouse ICOS (CD278) BUV395 (Clone 398.4A)	BD Biosciences	Cat# 565884
Anti-mouse CD69 BUV805 (Clone H1.2F3)	BD Biosciences	Cat# 741927
Anti-mouse KLRG-1 BUV661 (Clone 2F1)	BD Biosciences	Cat# 741586
Anti-mouse CD279 (PD-1) BUV737 (Clone RMP1-30)	BD Biosciences	Cat# 749306
Anti-mouse CD90.2 BU496 (Clone 30-H12)	BD Biosciences	Cat# 741047
Anti-mouse LPAM-1(a4b7) BV711 (Clone DATK32)	BD Biosciences	Cat# 740701
Anti-mouse CD103 BV786 (Clone M290)	BD Biosciences	Cat# 564322
Anti-mouse CD25 PE-Dazzle 594 (Clone PC61)	BD Biosciences	Cat# 562694
Anti-mouse CD3e PE-Cy5 (Clone 145-2C11)	BD Biosciences	Cat# 553065
Anti-mouse CD45.2 BUV737 (Clone 104)	BD Biosciences	Cat# 612778
Anti-mouse CD45 BUV496 (Clone 30-F11)	BD Biosciences	Cat# 749889
Anti-mouse IL33R/ST2 PE (Clone U29-93)	BD Biosciences	Cat# 566311
Anti-mouse CD90.2 BV605 (Clone 30-H12)	BioLegend	Cat# 140317
Anti-mouse CD45 BV510 (Clone 30-F11)	BioLegend	Cat# 103137
Anti-mouse CD45.2 FITC (Clone 104)	BioLegend	Cat# 109806
Anti-mouse CD45.1 Percp-Cy5.5 (Clone A20)	BioLegend	Cat# 110728
Anti-mouse CD4 PE-Texas Red (Clone RM4-5)	eBioscience	Cat # MCD0417
Anti-mouse CD8a PE-Texas Red (Clone 5H10)	eBioscience	Cat # MCD0817
Anti-mouse I-A/I-E AF-700 (Clone M5/114.15.2)	BioLegend	Cat# 107621
Anti-mouse TCRγδ Biotin (Clone GL3)	BioLegend	Cat# 118103
Anti-mouse Epcam Biotin (Clone G8.8)	BioLegend	Cat# 118203
Anti-mouse CD8α Biotin (Clone 53.67)	BioLegend	Cat# 100704
Anti-mouse CD3 Biotin (Clone 17A2)	BioLegend	Cat# 100243
Anti-mouse CD4 Biotin (Clone GK1.5)	BioLegend	Cat# 100404
Anti-mouse CD45 FITC (Clone 30-F11)	BioLegend	Cat# 103107
Anti-mouse NK1.1 PE-Cy7 (Clone PK136)	BioLegend	Cat# 108713
Anti-mouse CD19 FITC (Clone MB19-1)	BioLegend	Cat# 101505
Anti-mouse CD3e FITC (Clone 145-2C11)	BD Bioscience	Cat# 563061
Anti-mouse CD4 FITC (Clone H1 29.19)	BD Bioscience	Cat# 553651
Anti-mouse CD8a FITC (Clone 53-6.7)	BD Bioscience	Cat# 553031
Anti-mouse Gr1 FITC (Clone R86-8C5)	BD Bioscience	Cat# 553126
Anti-mouse Siglec-H FITC (Clone 551)	BioLegend	Cat# 129603
Anti-mouse B220 FITC (Clone RA3-6B2)	BD Bioscience	Cat#553088
Anti-mouse c-Kit/CD117 BV510 (Clone ACK2)	BioLegend	Cat#135119
Anti-mouse Sca-1 BV650 (Clone D7)	BioLegend	Cat#108143
Anti-mouse Flt3/CD135 PerCP–eFluor 710 (Clone A2F10)	eBioscience	Cat#46-1351-80
Anti-mouse CD27 PE (Clone LG3A10)	BD Bioscience	Cat# 558754
Anti-mouse Thy1.2 AF-700 (Clone 30-H12)	BioLegend	Cat# 105320
Anti-mouse ICOS BV785 (Clone C398.4A)	BioLegend	Cat# 313533
Anti-mouse IL18Rα AF647 (Clone BG/IL18RA)	BioLegend	Cat# 132903
Anti-mouse IL33R/ST2 (ST2) PE-Cy7 (Clone RMST2-2)	eBioscience	Cat#25-9335-82
Streptavidin BV510	Biolegend	Cat#405233
Anti-mouse IL25R/IL17RB PE (Clone 9B10)	BioLegend	Cat#146305
Anti-mouse CD127/IL7Rα BV421 (Clone A7R34)	BD Biosciences	Cat# 566377
Anti-mouse TCRγδ Antibody APC (Clone GL3)	BioLegend	Cat#118115
Anti-mouse/human CD11b FITC (Clone M1/70)	eBioscience	Cat# 11-0112-82
Anti-mouse TER-119 FITC (Clone TER-119)	eBioscience	Cat# 11-5921-82
Anti-human CD14 APC-eF780 (Clone 61D3)	eBioscience	Cat# 47-0149-42
Anti-human CD19 APC-eF780 (Clone HIB19)	eBioscience	Cat# 47-0199-42
Anti-human CD123 APC-eF780 (Clone 6H6)	eBioscience	Cat# 47-1239-42
Anti-human CD3 APC-eF780 (Clone SK7)	eBioscience	Cat# 47-0038-42
Anti-human FceRIa APC Vio770 (Clone CRA1)	Miltenyi	Cat# 130-105-500
Anti-human CD127 PE (Clone MB15-18C9)	Miltenyi	Cat# 130-113-410
Anti-human CD218(IL18Ra) PEVio770 (Clone H44)	Miltenyi	Cat# 130-101-723
Anti-human ICOS APC (Clone REA192)	Miltenyi	Cat# 130-101-723
Anti-human EOMES FITC (Clone WD1928)	eBioscience	Cat# 11-4877-42
Anti-human CRTH2 PerCP-Cy5.5 (Clone BM16)	BioLegend	Cat# 350116
Anti-mouse/human T-bet BV605 (Clone 4B10)	BioLegend	Cat# 644817
Anti-human CD117(c-KIT) BV650 (Clone 104D2)	BioLegend	Cat# 313222
Anti-human CD103 BV711 (Clone Ber-ACT8)	BioLegend	Cat# 350222
Anti-human CD336(NKP44) Biotin (Clone P44-8)	BioLegend	Cat# 325106
Streptavidin BV785	BioLegend	Cat# 405249
Experimental Models: Organisms/Strains
Mouse: wild-type:	C57BL/6, Charles River Laboratories
Mouse: CD45.1/Ly5.1	B6.SJL-Ptprca Pepcb/BoyJ, Jax mice	JAX stock #006584
Mouse: Ubiquitin tdT (“Ubow”)	(Ghigo et al., 2013)
Mouse: IL5cre x LSLtdTom	(Nussbaum et al, 2013)
Mouse: RAG1 -/-	B6.129S7-Rag1tm1Mom/J	JAX stock #002216
Mouse: RAG2-/- γc-/-	C57BL/6NTac;B10(Cg)-Rag2tm1Fwa Il2rgtm1Wj1, Taconic, 4111-F
Software and Algorithms
FlowJo v.10.5.0	FlowJo	https://www.flowjo.com
Prism v.6.0.	GraphPad	https://www.graphpad.com
R v. 3.5.1 and 3.6.1		https://www.r-project.org/