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. Author manuscript; available in PMC: 2022 Jan 19.
Published in final edited form as: Nat Immunol. 2021 Jul 19;22(9):1093–1106. doi: 10.1038/s41590-021-00968-4

Extended Data Fig. 9. RELB, IRF5, and JUNB affect neutrophil inflammatory production.

Extended Data Fig. 9

a) Cytokine-production-related gene expression in WT, Cebpβ–/–, RelB–/–, Irf5–/– or JunB–/– HoxB8 neutrophils.

b) Chemokine-production-related gene expression in Cebpβ–/–, RelB–/–, Irf5–/– or JunB–/– HoxB8 neutrophils.

c) Il1α, Il6 and Cxcl2 mRNA induction in HoxB8 neutrophils stimulated with zymosan (50ug/ml) for 0, 1, 2 hours. Gene expression was measured by qPCR. Data are shown as means and SD from three independent experiments. Significant differences compared between KO and WT neutrophils are denoted as: *P<0.05, **P<0.01, ****P<0.0001, and ****P<0.0001; (DM one-way ANOVA with Dunnett’s multiple comparisons test).

d) Cytokines and chemokines secreted from WT HoxB8 neutrophils challenged with Zymosan for 2h, measured by the proteome array shown in Fig. 6b.

e) Densitometric quantification of IL1β secretion from HoxB8 neutrophils stimulated with stimulated with zymosan (50ug/ml) for 2 hours, measured with 30 minutes exposure time. Data are shown as mean and SD of three biologically independent samples. Significant differences compared between KO and WT neutrophils are denoted as: *P<0.05, **P<0.01; (Ordinary one-way ANOVA with Dunnett’s multiple comparisons test).