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. Author manuscript; available in PMC: 2021 Dec 14.
Published in final edited form as: Nat Mater. 2021 Jun 14;20(9):1281–1289. doi: 10.1038/s41563-021-01020-4

Fig. 3. Shell yield and stability.

Fig. 3

(a) Laser-scanned fluorescent images of 0.5% agarose gels showing the assembly of octahedra, T=1, T=3 and T=4 shells at 40°C with a monomer concentration of 5 nM at different time points. Solid lines give cross-sectional lane intensity profiles from the 1d (octahedron, T=1) and 14d samples (T=3, T=4). sc: M13-8064 scaffold strand as reference. (b) Triangle exchange experiments. Cyan: FRET-pair labeled T=1 shells. Orange: unlabeled shells. Symbols give FRET signals measured at different incubation times in the presence of the indicated concentrations of Mg2+ for n=2 independent measurements (design details see Supplementary Fig. 44). (c) Negative-staining TEM image of octahedral shells coated with a 1:1 mixture of oligolysine and oligolysine-PEG and incubated for 1 h and 24h in 55% mouse serum at 37°C.