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. Author manuscript; available in PMC: 2021 Sep 21.
Published in final edited form as: J Mol Biol. 2016 Jan 11;428(10 Pt A):2011–24. doi: 10.1016/j.jmb.2016.01.002

Fig. 7. Half-bleach experiments of phase-separated FUS and FG-Nup100 assemblages (droplets).

Fig. 7

(a) Droplets were formed from GFP-labeled FUS, and then only the right-hand half of a droplet was bleached. The fluorescence recovered rapidly (blue to red indicating increasing fluorescence intensity), which points to the droplet being dynamic (liquid-like), and is the result of a liquid-liquid phase separation. (b) A similar half-bleach experiment from a different study for FG-Nup100 (doped with FG-Nup100 labeled with a synthetic fluorophore, shown in red). The droplets appear rigid, which points to the existence of a liquid-gel phase separation. Reprinted from Refs. [78] with permission from Elsevier and [12], respectively.