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. Author manuscript; available in PMC: 2021 Dec 21.
Published in final edited form as: Nat Cell Biol. 2021 Jun 21;23(7):745–757. doi: 10.1038/s41556-021-00699-6

Extended Data Fig. 3. Apicobasal distribution of F-actin and Myosin in organoid monolayers.

Extended Data Fig. 3

a-b, Apical, medial and basal projections of F-Actin (Phalloidin, a) and myosin IIA-eGFP (b). The stem cell compartment (Stem), the Transit amplifying zone (TA) and the villus-like domain (villus-like) are zoomed in the regions of the monolayer indicated with the respective colors. Representative images from 4 (a) and 2 (b) independent experiments. Scale bars, 20 μm. Stiffness of the gel, 5kPa.