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. Author manuscript; available in PMC: 2021 Dec 21.
Published in final edited form as: Nat Cell Biol. 2021 Jun 21;23(7):745–757. doi: 10.1038/s41556-021-00699-6

Extended Data Fig. 5. Effect of myosin inhibition in organoid cell shape.

Extended Data Fig. 5

a-b, 3D segmentation of a crypt on 15 kPa gels under baseline conditions (a) and the same crypt after 3h treatment with 15μM of blebbistatin (b). Top: medial view. Bottom: lateral view. Representative images of 2 independent experiments. Scale bar, 20 μm. c-d, Apicobasal tilt (c) and basal aspect ratio (d) as a function of the distance to crypt center on rigid substrates (15 kPa) before and after blebbistatin. Vertical dashed line indicates the boundary between the stem cell compartment and the transit amplifying zone. From center to edge bins, n = 42, 72, 150 and 232 cells for baseline crypt and 39, 82, 131 and 209 for blebbistatin treatment. Data from 2 independent experiments.