Table 1. Rate limiting steps in the early HBV life cycle.
| HepG2-NTCP K7 | HepG2 | |||||
|---|---|---|---|---|---|---|
| Absolute copy number (per cm2) | % From input virus | % From attached virus | Absolute copy number (per cm2) | % From input virus | % From attached virus | |
| HBV inoculum | 1.5 × 108 ± 2.9 × 107 | − | − | 1.5 × 108 ± 2.9 × 107 | − | − |
| Attachment | 3.7 × 107 ± 4.7 × 106 | 25 ± 6 | − | 2.6 × 107 ± 4.1 × 106 | 17 ± 5 | − |
| Total intracellular HBV DNA (6 h p.i) | 3.1 × 107 ± 2.5 × 106 | 21 ± 6 | 84 ± 21 | 1.2 × 107 ± 1.9 × 106 | 8±3 | 46 ± 17 |
| cccDNA (72 h p.i.) | 2.2 × 105 ± 3.8 × 104 | 0.15 ± 0.05 | 0.6 ± 0.16 | − | − | − |
Note:Summary of synchronised infection in HepG2-NTCP and HepG2 cells. Data showing input HBV DNA of the inoculum, cell-associated HBV DNA (attachment) and intracellular HBV DNA after 6 h post infection (p.i.) at 37°C along with cccDNA levels at 72 h p.i. Absolute copy numbers are presented from cells/cm2. These data are presented as the mean ± SEM of eight independent experiment with biological duplicates. n.d: not detected.