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. Author manuscript; available in PMC: 2021 Sep 26.
Published in final edited form as: Cell Microbiol. 2020 Sep 28;22(12):e13250. doi: 10.1111/cmi.13250

Table 1. Rate limiting steps in the early HBV life cycle.

HepG2-NTCP K7 HepG2
Absolute copy number (per cm2) % From input virus % From attached virus Absolute copy number (per cm2) % From input virus % From attached virus
HBV inoculum 1.5 × 108 ± 2.9 × 107 − − 1.5 × 108 ± 2.9 × 107 − −
Attachment 3.7 × 107 ± 4.7 × 106 25 ± 6 − 2.6 × 107 ± 4.1 × 106 17 ± 5 −
Total intracellular HBV DNA (6 h p.i) 3.1 × 107 ± 2.5 × 106 21 ± 6 84 ± 21 1.2 × 107 ± 1.9 × 106 8±3 46 ± 17
cccDNA (72 h p.i.) 2.2 × 105 ± 3.8 × 104 0.15 ± 0.05 0.6 ± 0.16 − − −

Note:Summary of synchronised infection in HepG2-NTCP and HepG2 cells. Data showing input HBV DNA of the inoculum, cell-associated HBV DNA (attachment) and intracellular HBV DNA after 6 h post infection (p.i.) at 37°C along with cccDNA levels at 72 h p.i. Absolute copy numbers are presented from cells/cm2. These data are presented as the mean ± SEM of eight independent experiment with biological duplicates. n.d: not detected.