Extended Data Table 1. Cryo-EM data collection, refinement and validation statistics.
| A1/2_γ8/C2 Resting state | A1/2_γ8 Resting state | A1/2_γ8/C2 Active state | ||
|---|---|---|---|---|
| LBD-TMD (EMDB-12805) (PDB 7OCE) | NTD (EMDB-12803) (PDB 7OCC) | LBD-TMD (EMDB-12804) (PDB 7OCD) | LBD-TMD (EMDB-12806) (PDB 7OCF) | |
| Data collection and processing | ||||
| Microscope | FEI Titan Krios | FEI Titan Krios | ||
| Detector | K3 + GIF | K3 + GIF | ||
| Magnification | 81000X | 81000X | ||
| Voltage (kV) | 300 | 300 | ||
| Electron exposure (e-/Å2) | 50 | 50 | ||
| Defocus range (μm) | -1.2 to -2.4 | -1.2 to -2.4 | ||
| Pixel size | 1.07 | 1.07 | ||
| Symmetry imposed | C2 | C2 | ||
| Micrographs | 10462 | 8246 | ||
| Map resolution (Å) | 3.1 | 3.4 | 3.5 | 3.6 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
| Refinement | ||||
| Initial model used (PDB) | 6QKC | 6QKC | 6QKZ | 6QKC |
| Model resolution (Å) | 3.1 | 3.4 | 3.5 | 3.5 |
| FSC threshold | 0.5 | 0.5 | 0.5 | 0.5 |
| Map sharpening B factor (Å2) | -113 | -142 | -115 | -122 |
| Model composition | ||||
| Non-hydrogen atoms | 18092 | 11202 | 13624 | 17092 |
| Protein residues | 2322 | 1480 | 1920 | 2298 |
| Ligands | NBQX: 4 | NAG: 18, BMA: 2 | NBQX: 4 | CTZ: 4 |
| Lipids | 48 | 0 | 6 | 26 |
| B factors (Å2) | ||||
| Protein | 37.44 | 34.10 | 61.09 | 50.60 |
| Ligand | 35.48 | 59.58 | 48.78 | 31.70 |
| R.m.s. deviations | ||||
| Bond lengths (Å) | 0.006 | 0.010 | 0.007 | 0.007 |
| Bond angles | 0.684 | 0.722 | 0.595 | 0.616 |
| Validation | ||||
| Molprobity score | 1.45 | 1.46 | 1.75 | 1.54 |
| Clashscore | 4.07 | 5.04 | 7.03 | 4.84 |
| Poor rotamers (%) | 0 | 0 | 0 | 0 |
| Ramachandran plot | ||||
| Favoured (%) | 96.22 | 96.87 | 94.73 | 95.79 |
| Allowed (%) | 3.78 | 3.13 | 5.27 | 4.21 |
| Disallowed (%) | 0 | 0 | 0 | 0 |