Extended Data Fig. 8. Hobit regulates the effector differentiation of Eomes⁺CD49a⁺ group 1 ILCs, and phenotype of ILC1 in Treg-cell depleted mice.

a-e FACS analysis of Eomes⁺CD49a⁺ tissue-resident NK1.1⁺ cells in salivary glands of WT and Hobit^KO mice. a Representative FACS analysis of Hobit^Tom expression within ILC1 (CD49b⁻ CD49a⁺), Eomes⁺CD49a⁺NK1.1⁺ cells (identified by gating as CD49b⁺CD49a⁺, to avoid permeabilization for the analysis of the Hobit^Tom reporter signal) and cNK cells (CD49b⁺CD49a⁻) within Lin NK1.1’ cells. b Absolute numbers of Eomes⁺CD49a⁺NK1.1⁺ cells. c Representative FACS analysis showing indicated marker expression. d Frequency of marker-positive cells. e gMFI of TCF-1 and IL-18R1 expression within marker-positive cells. Data are representative of 3 independent experiments with n=3 mice per group. f Representative FACS plots show expression of indicated proteins of ILC1s in kidneys, mesenteric lymph nodes and small intestine lamina propria of FoxP3^DTR mice on d8 of Treg cell depletion (DTx). Data are representative of 2-3 independent experiments with n=4 mice per group. Bar graphs indicate individual mice (symbols) and mean (bar), error bars display means ± SD. Statistical significance was calculated by unpaired two-tailed t-test; **p < 0.01, ***p < 0.001, ns – not significant.