Fig. 8. The developmental potential of ILC1s is conserved across tissues.

a-fIn vitro culture of CD127⁺ kidney (a-c) and small intestinal (d-f) ILC1 in the presence of IL-2 and OP9-DL1 feeder cells. a, d Representative FACS plots on d7. b, e Absolute numbers of cells on d0 (input=400 cells) and d7. c, f Frequency of CD127⁺, CD3γ⁺ and Gzma⁺Gzmb⁺ cells. Data representative of 3 independent experiments with n=3 replicates per experiment. g FACS analysis of ILC1s from indicated organs of Treg depleted or mock treated FoxP3^DTR mice on d8 of DT treatment (DTx). Histograms indicate the frequency of ILC1s expressing indicated proteins is depicted. Data are representative of 3 independent experiments with n=4 mice per group. h Schematic summarizing sequential mechanisms of ILC1 lineage commitment and effector differentiation downstream of TCF-1^hi “early” ILC1s, which is transcriptionally regulated by Hobit. ILC1s across tissues have a uniform capacity to differentiate towards TCF-1^lo Gzm^hi cells. Bar graphs indicate replicates (b, c, e, f) or individual mice (g) (symbols) and mean (bar), error bars display means ± SD. Statistical significance was calculated by unpaired two-tailed t-test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.