Skip to main content
. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: Eur J Cell Biol. 2010 Nov 1;90(1):26–36. doi: 10.1016/j.ejcb.2010.09.008

Figure 1. Serum stimulation promotes local matrix deformation and degradation during contraction.

Figure 1

Cells were seeded in collagen matrices in medium containing 10% FBS, and images of fixed (HTF5003, 3T3) or live (HDF, HSF) gels were obtained using confocal reflection microscopy at different times after matrix polymerisation. HTF and 3T3 induced a rapid marked pericellular alignment of the collagen fibres (arrows), while matrix degradation could be seen either in the form of voids around the cell (HSF, arrowheads) or as a general fading of the matrix (t24hr for HDF, t120 hours in HTF and 3T3). Phase images background was filtered for clarity using Photoshop. Bars, 20 μm. Images shown are representative of 3 independent experiments.