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. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: Eur J Cell Biol. 2010 Nov 1;90(1):26–36. doi: 10.1016/j.ejcb.2010.09.008

Figure 3. MMP inhibition affects matrix remodelling and gel contraction. Fibroblasts seeded in gels were incubated for 7 days in the presence of 10% FBS with or without 100 μM of MMP inhibitor GM6001.

Figure 3

A) Matrix contraction in presence of 10% FBS (filled circles), 10% FBS plus 100 μM GM6001 (filled squares), serum free medium with 0.7% BSA (empty circles) and 10% FBS plus 0.5% DMSO (empty squares). Shown is mean ± SEM, from 4 (HTF, HSF) and 6 (3T3) sets of triplicate matrices. B) MMP inhibition affects cell-matrix interaction. HTF seeded in collagen gels in presence of 10% serum plus 100 μM GM6001 showed delayed pericellular collagen fibre alignment (arrows) and better preservation of the fibre network (compare to Fig. 1). Scale bar, 20 μm.