Extended Data Figure 8. Relationship between decreased ovarian reserve and gene expression.

Open bar/dot groups – control maternal diet, normal ovarian reserve. Grey bar/dot groups: obesogenic maternal diet, reduced ovarian reserve. a, Ovarian follicular reserve in young adulthood in wild-type mice. Total follicles/mm³ ovarian tissue at 12 weeks. Dots: individual observations. Bar heights and error bars: mean± SEM. n= 8 biologically independent animals from different litters in each group. P=0.0091 derived from 2-way ANOVA after correction for multiple hypothesis testing. b, Brsk1 expression in the same animals, measured using qrtPCR and expressed as average copy number. Dots: individual observations. Bar heights and error bars: mean± SEM. n= 8 biologically independent animals from different litters in each group. P=0.0001 derived from 2-way ANOVA after correction for multiple hypothesis testing. c, Wee1 expression in the same animals, measured using qrtPCR and expressed as average copy number. Dots: individual observations. Bar heights and error bars: mean± SEM. n= 8 biologically independent animals from different litters in each group. P=0.0256 derived from 2-way ANOVA after correction for multiple hypothesis testing. d, Dmc1 expression in the same animals, measured using qrtPCR and expressed as average copy number. Dots: individual observations. Bar heights and error bars: mean± SEM. n= 8 biologically independent animals from different litters in each group. P=0.00001 derived from 2-way ANOVA after correction for multiple hypothesis testing. e, Mapt expression in the same animals, measured using qrtPCR and expressed as average copy number. Dots: individual observations. Bar heights and error bars: mean± SEM. n= 8 biologically independent animals from different litters in each group. P=0.0378 derived from 2-way ANOVA after correction for multiple hypothesis testing. qrtPCR, quantitative reverse transcription polymerase chain reaction; SEM, standard error of mean. *, P<0.05; **, P<0.01; ***, P<0.001.