Skip to main content
. Author manuscript; available in PMC: 2021 Oct 14.
Published in final edited form as: Adv Funct Mater. 2020 Jun 8;30(48):2000893. doi: 10.1002/adfm.202000893

Figure 3. Organoids in PIC retain a stem/progenitor phenotype and are highly proliferative.

Figure 3

Single organoid cells were seeded at day 0 and organoids were expanded in the different hydrogels for 14 days. Three different donors were analyzed in independent experiments (N = 3). a) Immunofluorescent analysis of paraffin-embedded organoids confirmed that the organoids in all gels have an epithelial progenitor phenotype and are highly proliferative. Human liver tissue was used as a control. Whole transcriptome sequencing analysis of organoids from two independent donors at day 7 of expansion in Matrigel, 1k PIC-LEC, and 5k PIC-LEC (N = 2). b) Pearson correlation map of the organoids in different hydrogels. Note that the correlation of cultured organoids is more than 99%. c) Heatmap of markers for stem/progenitor cells, epithelial cells, and mature hepatocytes selected from the mRNA sequencing data.