Figure 6. Human recombinant laminin-111 can substitute LEC.

a) Organoids from three different donors were cultured for 7 days in Matrigel, PIC-plain, or 1k PIC supplemented with either LEC or human recombinant Laminin (hrlaminin-111). Light microscopy pictures show a similar morphological phenotype in LEC and hrlaminin-111, with increased organoid size and number at higher laminin concentrations. (N = 3) b) Immunofluorescent analysis of paraffin-embedded organoids confirmed that the organoids in all gels have an epithelial progenitor phenotype and are highly proliferative. Organoids cultured in Matrigel, 5k PIC with 3 mg mL⁻¹ LEC or 5k PIC with 2 mg mL⁻¹ hrlaminin-111 are displayed. Human liver tissue was used as a control (N = 1).