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. Author manuscript; available in PMC: 2022 Mar 2.
Published in final edited form as: Nat Cell Biol. 2021 Sep 2;23(9):953–966. doi: 10.1038/s41556-021-00742-6

Extended Data Fig. 4. Pharmacological inhibition of Notch activity by DAPT treatment enhances the differentiation of secretory cells into AT2 cells during injury repair.

Extended Data Fig. 4

a, Experimental design of lineage-tracing analysis of Scgb1a1-CreERTM/+;R26RtdTomato/+ mice after bleomycin injury. DMSO or DAPT (50mg/kg body weight) was administrated via intraperitoneally as indicated time points. b, Representative IF images showing the increased Scgb1a1+ lineage-labelled Act-Tub+ ciliated cells in DAPT-treated mouse lungs at day 21 post bleomycin injury compared to DMSO control mice. Tomato (for Scgb1a1 lineage, red), Acetylated Tubulin (Act-Tub, white), and DAPI (blue). Scale bar, 50μm. c,d, Representative IF images showing the derivation of Scgb1a1+ lineage-labelled SPC+ AT2 (left) or CC10+ secretory cells (right) in DMSO control (c) or DAPT-treated (d) mice at day 21 post bleomycin injury. Tomato (for Scgb1a1 lineage, red), SPC (white), CC10 (green), and DAPI (blue). Scale bar, 100μm.