Porcine conjunctiva tissue samples remain viable over time. A, Samples were weighed preceding each experiment to ensure that the sample size remained constant. No significant variations in tissue size were found between conditions. B, Cell cytotoxic effects were minimal for all conditions. GM6001 did not increase lactate dehydrogenase levels released into the extracellular medium surrounding the samples. Media samples collected throughout the 4-week period were measured for lactate dehydrogenase activity at different time points. The data were combined to give overall percentage lactate dehydrogenase release, as no differences between the time points were seen. C, Three-dimensional projection shows cells stained with the vital dye carboxyfluorescein diacetate following 4 weeks in culture in the presence of GM6001. Data represent the mean (SEM) (n=3). SF indicates serum free; FBS, fetal bovine serum; FGM, FBS + GM6001; TGF, transforming growth factor β2; TGM, TGF plus GM6001; and TOT, total lactate dehydrogenase release.