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. Author manuscript; available in PMC: 2021 Oct 20.
Published in final edited form as: Arch Ophthalmol. 2011 May 1;129(5):619–27. doi: 10.1001/archophthalmol.2011.91

Figure 8.

Figure 8

Matrix metalloproteinase inhibitor GM6001 (GM) prevents matrix degradation in rabbit conjunctiva. Images were take of live rabbit conjunctiva using reflection confocal microscopy (red) to observe matrix conditions at the end of the ex vivo contraction assay. Live cells (green) were visualized using the cell vital dye carboxyfluorescein diacetate. The upper panel shows 3-dimensional reconstruction of z-stack confocal images, and the lower panel shows a flat projection of all sections. After 4 weeks in fetal bovine serum (FBS), no fiber degradation is observed. However, fiber degradation is seen in tissue exposed to 10 ng/mL transforming growth factor β2 (TGFβ2), and this effect is inhibited by GM6001 (TGFβ2 + GM). Viable cells are present in the segments in all conditions. Bar indicates 100 μm.