a, In mismatch repair-deficient strains (msh2Δ) gap repair may generate segments of 6:2 marker segregation flanked by ‘trans’ 5:3 hDNA. b, Quantification of meiotic recombination event types in control (msh2Δ). c, Representative categorisations of 6:2-containing events based on flanking hDNA (see also Extended Data Fig.7). Horizontal bars are sequence polymorphism calls (red or blue) from meiotic octads. 6:2 segments indicated in pale blue. Lower panels are smoothed histograms of total Spo11-oligo and Spo11-DC signals. d, As in (b), but for pooled ‘exo-mlhΔ msh2Δ’ (exo1Δ msh2Δ, mlh1Δ msh2Δ, mlh3Δ msh2Δ) strains. e, Frequency of 6:2 segments per event for each category. f, Fraction of 6:2 segments (in msh2Δ) overlapping annotated hotspots. P values indicate two-tailed Z-test of proportions relative to msh2Δ (e), or between categories (f). In (e-f) whiskers are 95% confidence intervals; n=9 (msh2Δ) or 6 (exo-mlhΔ) biologically independent samples.