Extended Data Table 1. Strains used in this study.
Unless indicated, all strains are of the SK1 genetic background46. For recombination mapping in octads, haploids were mated directly prior to sporulation as described19,34. The specific lys2 mutation is not known, but has been historically present in SK1 background strains. The ura3 allele contains an insertion of a Ty transposon. When not directly indicated, the arg4 mutant allele is either arg4-nsp or arg4-bgl. When not directly indicated, the leu2 mutant allele is either leu2::hisG or leu2Δ allele. We have no indication that allelic differences at these loci have any influence on the key observations reported in this study. lys2, ura3, arg4, and leu2 alleles all confer lack of growth on drop-out media lacking the relevant component.
| Strain name | Genotype | Origin |
|---|---|---|
| VG296 | MATa/alpha, ho::LYS2/”, lys2/”, ura3/”, arg4/", leu2::hisG/”, his4X::LEU2,/” nuc1::LEU2/”, SPO11-His6-FLAG3-loxP-KanMX-loxP/” | This study |
| VG303 | MATa/alpha, ho::LYS2/", lys2/”, ura3/”, arg4/”, leu2/”, his4X::LEU2,/” nuc1::LEU2/”, SPO11-His6-FLAG3-loxP-KanMX-loxP/”, sae2Δ::KanMX6/” | This study |
| VG300 | MATa/alpha, lys2/”, ura3/”, arg4/”, leu2/”, his4X::LEU2,/” nuc1::LEU2/”, SPO11-His6-FLAG3-loxP-KanMX-loxP/”, tel1Δ::HphMX4/” | This study |
| VG302 | MATa/alpha, lys2/”, ura3/”, arg4/”, leu2/”, his4X::LEU2,/” nuc1::LEU2/”, SPO11-His6-FLAG3-loxP-KanMX-loxP/”, tel1Δ::HphMX4/”, sae2Δ::KanMX6/” | This study |
| SKY 3935 | MATα; ho::LYS2; lys2; ura3; leu2::hisG; his3::hisG; SPO11-5ProA-his5+sp | Mohibullah et al, 2017 |
| SKY 3934 | MATα; ho::LYS2; lys2; ura3; leu2::hisG; his3::hisG; SPO11-5ProA-his5+sp | Mohibullah et al, 2017 |
| SKY 3950 | MATα; ho::LYS2; lys2; ura3; leu2::hisG; his3::hisG; SPO11-5ProA-his5+sp; tel1Δ::kanMX | Mohibullah et al, 2017 |
| SKY 3951 | MATα; ho::LYS2; lys2; ura3; leu2::hisG; his3::hisG; SPO11-5ProA-his5+sp; tel1Δ::kanMX | Mohibullah et al, 2017 |
| MC26 | MATα ho::LYS2 lys2Δ ura3Δ arg4 leu2 msh2Δ::KanMX (SK1) | Crawford et al, 2018 |
| MC49 | MATα; ade8Δ msh2Δ::KanMX (S288c) | Crawford et al, 2018 |
| MC30 | MATα ho::LYS2 lys2Δ ura3Δ arg4 leu2 msh2Δ::KanMX tel1Δ::HphMX4 (SK1) | This study |
| MC53 | MATα; ade8Δ msh2Δ::KanMX tel1Δ::HphMX4 (S288c) | This study |
| BLY727 | MATα, msh2::HPH, mlh1::KanMX6 (SK1) | Marsolier-Kergoat et al 2018 |
| BLY723 | MATα, msh2::HPH, mlh1::KanMX6 (S288c) | Marsolier-Kergoat et al 2018 |
| BLY372 | MATα, msh2::HPH, mlh3::KanMX6 (SK1) | Marsolier-Kergoat et al 2018 |
| BLY365 | MATα, msh2::HPH, mlh3::KanMX6 (S288c) | Marsolier-Kergoat et al 2018 |
| BLY912 | MATα, msh2::HPH, exo1::KanMX4 (SK1) | Marsolier-Kergoat et al 2018 |
| BLY1070 | MATα, msh2::HPH, exo1::KanMX4 (S288c) | Marsolier-Kergoat et al 2018 |