Fig. 5. Orthogonal in vivo RNA editing validates the OTP86^DYW domain structure and activation.

a, The C-to-U editing activities at the nad4eU272SL site in E. coli expressed with the PPR56^PPRE1E2-OTP86^DYW fusion protein (OTP86DYW), or its mutants, are plotted. The activities of mutants are relative to that of PPR56^PPRE1E2-OTP86^DYW (82.4 ± 2.1% edited). The bars represent the mean values, with each mutant protein ±s.d. based on three independent experiments (shown as yellow diamonds). The soluble protein expression of each mutated construct in E. coli was verified by western blot analysis (Supplementary Fig. 7). b, The activities of OTP86^DYW mutants shown in a plotted on the surface of the inactive OTP86^DYW and the activated OTP86^DYW★ structure as a heatmap (activity is scaled in the bar on the bottom), with untested residues shown in grey.