Extended Data Fig. 4. Flow cytometry gating strategy to distinguish uncaged and caged CD8⁺ T cells.

Data depict gating strategy used for flow cytometry analysis as well as for index sorting of uncaged CD8⁺ T cells. Viable single CD8⁺ T cells are selected based on double staining with αCD8^D-1-PsT and αCD8^D-2-FITC (serving as a stable signal to identify cells after loss of the AF594-positive cage). Uncaged cells are distinguished from caged cells based on their elevated αFLAG signal and reduced αCD8^D-1-PsT signal (AF594^low αFLAG^high).