Figure 1. CD34⁺ cells from CMML or sAML donors engraft in NSGS mice.

A Purified CD34⁺ cells from 4 donors (numbers refer to Supplementary Table S2A) were injected i.v. into sublethally irradiated NSGS mice as described in the text. After 8-12 weeks, mice were sacrificed and engraftment assessed by flow cytometry of BM cells using antibodies specific for human CD33 and CD45. A single representative mouse for each donor is shown. B,C T-cell depleted bulk MNC or purified CD34⁺ or CD34^– cells from donors with CMML (B) or sAML (C) were injected into NSGS mice. Bars show the mean±SD of engraftment levels (i.e. percentage of human CD45⁺/CD33⁺/CD14⁺ cells) in all mice in each cohort. **, p<0.01 D Cytospin preparations of BM cells obtained from mice were stained using Wright-Giemsa stain. The figure shows a photograph from one representative mouse in each cohort. E BM cells from one sAML recipient mouse were sequentially injected into secondary recipient mice. A representative dot-plot demonstrating the engraftment in the primary recipient and secondary recipients is shown. Abbreviations: CMML, chronic myelomonocytic leukemia; sAML, post-CMML secondary acute myeloid leukemia; MNC, mononuclear cells.