Table 2. Surface phenotype of LSC in CMML, sAML, and healthy hematopoietic stem cells.
| Antigen | CD | Expression on the cell surface of CD34+/CD38– cells in | ||
|---|---|---|---|---|
| NBM | CMML | sAML | ||
| IL-2RA | 25 | – | – | ++ |
| DPPIV | 26 | – | – | – |
| Siglec-3 | 33 | ++ | ++ | ++ |
| IAP | 47 | ++ | ++ | ++ |
| Campath-1 | 52 | +/– | +/– | +/– |
| Thy-1 | 90 | +/– | – | – |
| C1QR1 | 93 | +/– | + | + |
| TACTILE | 96 | – | – | – |
| Endoglin | 105 | + | + | + |
| TPO-R | 110 | +/– | +/– | + |
| G-CSFR | 114 | – | – | +/– |
| M-CSFR | 115 | – | – | – |
| GM-CSFR | 116 | +/– | +/– | +/– |
| KIT | 117 | ++ | ++ | + |
| IL-3RA | 123 | + | + | ++ |
| AC133 | 133 | ++ | ++ | ++ |
| FLT-3 | 135 | +/– | +/– | + |
| CXCR4 | 184 | – | +/– | +/– |
| IGF1R | 221 | +/– | +/– | +/– |
| LNGFR | 271 | – | – | – |
| PD-L1 | 274 | + | +/– | +/– |
| KDR | 309 | – | – | – |
| CLL-1 | 371 | – | – | +/– |
| IL-1RAP | n.c. | – | +/– | + |
| EPO-R | n.c. | +/– | – | +/– |
| OSMRb | n.c. | – | – | – |
BM samples from patients with CMML (n=20), sAML (n=6) or healthy control samples (n=11) were analyzed by flow cytometry for expression of the indicated markers on the surface of CD34+/CD38– cells. Results are expressed as staining index (SI; median fluorescence intensity of the marker divided by median fluorescence intensity of the corresponding isotype control) and indicate the mean SI of all patients tested in the respective cohort. SI-dependent score of staining results: –, SI<1.5; +/–, SI 1.5-3; +, SI 3-10; ++, SI >10. Abbreviations: NBM, normal bone marrow, CMML, chronic myelomonocytic leukemia; sAML, secondary AML from CMML; IL-2RA, interleukin-2 receptor alpha chain; DPPIV, dipeptidylpeptidase IV; IAP, integrin-associated protein; C1QR1, Complement activator C1q receptor 1; TACTILE, T-cell activation increased late expression; TPO-R, thrombopoietin receptor; G-CSFR, granulocyte-colony stimulating factor receptor; M-CSFR, macrophage-colony stimulating factor receptor; GM-CSFR, granulocyte-macrophage colony stimulating factor receptor; IL-3RA, interleukin-3 receptor alpha chain; FLT-3, fms-like tyrosine kinase 3; CXCR4, chemokine C-X-C motif receptor 4; IGF1R, insulin-like growth factor 1 receptor; LNGFR, low affinity nerve growth factor receptor; PD-L1, programmed death ligand 1; KDR, kinase insert domain receptor; CLL-1, C-type lectin like 1; IL-1RAP, interleukin-1 receptor accessory protein; EPO-R, erythropoietin receptor; OSMRb, oncostatin M receptor beta chain.