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. Author manuscript; available in PMC: 2021 Oct 31.
Published in final edited form as: Cell Motil Cytoskeleton. 2006 Jul 1;63(7):395–414. doi: 10.1002/cm.20131

Figure 8. The Arp2/3-mediated in situ polymerisation is minimal further back from the edge even after 5 min.

Figure 8

A polymerisation mix containing actin plus Arp2/3 plus VCA (“actin + activated Arp2/3”) was incubated on extracted cytoskeleton for 1 (a) or 5 (c) min before the cells were fixed and processed for replica microscopy. Arrowheads pointing at gold particles illustrate the minimal incorporation of exogenous actin, even after 5 min polymerisation (b). Arrows point at typical unlabelled free-end filaments. Bar, 100 nm.