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. Author manuscript; available in PMC: 2021 Oct 31.
Published in final edited form as: Cell Motil Cytoskeleton. 2006 Jul 1;63(7):395–414. doi: 10.1002/cm.20131

Figure 9.

Figure 9

The Arp2/3-mediated in situ polymerisation pattern partially matches the barbed end pattern both spatially and temporally. MTln3 cells, starved (a,c) or stimulated with EGF for 1 min (b,d) were permeabilised with triton and stained for barbed end distribution (a,b) or Arp2/3-mediated in situ polymerisation (c,d). The barbed end distribution was assessed after 1 min incorporation of biotin labelled actin in standard polymerisation-enabling buffer, while the Arp2/3-mediated in situ polymerisation pattern was revealed after 5 min incubation of an actin/Arp2/3/VCA polymerisation mix. The Arp2/3-mediated in situ polymerisation pattern was similar in resting and stimulated cells and matched the barbed end distribution in stimulated cells. Exogenous actin accumulation around the cell circumference also appeared increased in stimulated cells as opposed to starved cells.