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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: J Clin Endocrinol Metab. 2009 Apr 7;94(7):2551–7. doi: 10.1210/jc.2009-0159

Fig. 4.

Fig. 4

A, Schematic representation of the SUR1(1-196) and SUR1(1-288) constructs, showing transmembrane helices and an N-terminal FLAG tag. B and C, Representative KATP currents recorded in response to successive voltage ramps from −110 to +100 mV in an inside-out patch excised from a Xenopus oocyte coexpressing the indicated homozygous Kir6.2ΔC construct in the presence of SUR1(1–196) (B) and/or SUR1(1-288) (C). The dashed line indicates the zero current level. D and E, Mean relationship between [ATP] and Kir6.2ΔC conductance (G), expressed relative to the conductance in the absence of nucleotide (Gc), for Kir6.2ΔC (black filled circles) and homKir6.2ΔC-28Δ32 (unfilled circles) channels in the presence of either SUR1(1-196) (D) or SUR1(1-288) (E). The lines are the best fit of the Hill equation to the mean data: D, WT, IC50 = 311 μM, n = 6; hom28Δ32, IC50 = 325 μM, n = 7. E, WT, IC50 = 136 μM, n = 5; hom28Δ32, IC50 = 202 μM, n = 5. Figures are derived from the fit of the mean data.