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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: J Clin Endocrinol Metab. 2009 Apr 7;94(7):2551–7. doi: 10.1210/jc.2009-0159

Fig. 6.

Fig. 6

Coimmunoprecipitation assays performed on oocytes expressing FLAG-tagged SUR1(1-196) and either HA-tagged Kir6.2ΔC or HA-tagged Kir6.2ΔC- 28Δ32. A, Top, Binding of Kir6.2ΔC and Kir6.2ΔC-28Δ32 to TMD0 [SUR1(1-196), n = 6], expressed as a percentage of binding to Kir6.2ΔC. Bound protein levels were normalized to the expression levels of SUR1(1-196). Bottom, upper panel, Representative anti-FLAG Western blots from individual experiments, showing SUR1(1-196) binding to Kir6.2ΔC and Kir6.2ΔC-28Δ32 (corresponding to the graph above). Bottom, lower panel, Representative Western blots of the oocyte lysate inputs into the binding assay, showing expression levels of TMD0. B, Top, Binding of Kir6.2ΔC and Kir6.2ΔC-28Δ32 to SUR1(1-288) (n = 4), expressed as a percentage of the binding of Kir6.2ΔC. **, P < 0.01 compared with Kir6.2ΔC. Bound protein levels were normalized to the precipitated levels of SUR1(1-288) and expression levels of Kir6.2ΔC. Bottom, upper panel, Representative anti-HA Western blots from individual experiments, showing Kir6.2ΔC or Kir6.2ΔC- 28Δ32 binding to SUR1(1-288), corresponding to the graph above. Bottom, lower panel, Representative Western blots of the oocyte lysate inputs into the binding assay, showing expression levels of Kir6.2ΔC or Kir6.2ΔC-28Δ32.