Figure 1.

A) A schematic of the Type I-C cas gene operon and CRISPR array. The surveillance complex is made up of Cas proteins (Cas5₁:Cas8₁:Cas7₇) and one crRNA, which recruits Cas3 upon target DNA recognition. Cas3 then degrades DNA through its dual helicase-nuclease activity. B) Growth curves of 2 PAO1^IC strains expressing different crRNAs targeting phzM (green and orange) compared to a non-targeting strain (blue). Values are the mean of 8 biological replicates each, error bars indicate SD values. C) Cultures resulting from phzM targeting, in the absence of inducer (-ind), presence (+ind), and after recovery. D) Whole-genome sequencing of threePAO1^ICself-targeted survivor strains. Bars indicate boundaries of deletions with ORF indicated below; red arrow indicates genomic position of targeted sequences.