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. Author manuscript; available in PMC: 2021 Nov 10.
Published in final edited form as: Nat Neurosci. 2020 Aug 3;23(10):1229–1239. doi: 10.1038/s41593-020-0679-6

Extended Data Fig. 3. CNO application itself during learning had no effect on remote memory (Related to Figure 1).

Extended Data Fig. 3

(A) Bilateral double injection of AAV8-GFAP-eGFP resulted in eGFP expression in CA1 astrocytes only. Scale bar – left 300μm, right 50 μm. Mice expressing eGFP in their CA1 astrocytes were injected with either Saline (n=6) or CNO (n=7) 30min before fear conditioning acquisition. CNO administration before training to eGFP-expressing mice had no effect on baseline freezing or recent contextual memory recall one day later (B). Neither did CNO have any effect on remote memory 20 days later or 45 days after that (C). In the non-associative place recognition test, CNO application before a first visit to a new environment had no effect on remote memory 28 days later (D), reflected by a similar decrease (p<0.0001) in the exploration between Saline injected (n=6) and CNO-treated mice (n=7) Example exploration traces and the average change (Δ) following treatment are shown on the right. Data presented as mean ± SEM.