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. Author manuscript; available in PMC: 2021 Nov 19.
Published in final edited form as: Science. 2021 Nov 11;374(6569):874–879. doi: 10.1126/science.abj3125

Fig. 4. Patronin localisation is defined by fusome and by a positive feed back loop of Dynein mediated transport.

Fig. 4

(A-B) Patronin associates with the fusome in a microtubule-dependent manner. Untreated (A) or colcemid-treated (B) live germaria expressing Patronin-YFP and Hts-Cherry. Regions 2a and 2b are shown as close-ups. (C) Shot links Patronin to the fusome. Live germaria containing wild type (WT; left) and shot mutant (right) cysts expressing Patronin-YFP either untreated (top) or treated with colcemid (bottom). (D) Patronin localisation depends on Dynein activity. Wild type (WT; top) and egalitarian mutant (bottom) live germaria expressing transgenic Patronin-GFP. (E) A diagram showing the 4 steps in cyst polarization that lead to the specification of the oocyte and its subsequent positioning at the posterior of the cyst in region 3. See text for details. Asterisk indicates the presumptive oocyte.